Figure 3: Impaired angiogenesis in Cys17Ser PKARIα ‘redox-dead’ KI mice.
From: Deficient angiogenesis in redox-dead Cys17Ser PKARIα knock-in mice
(a)The removal of the femoral artery severely restricted blood flow within the hind limb of KI and littermate WTs as monitored using laser Doppler. Over the subsequent 28 days, there was a time-dependent return of blood flow to the hind limb of wild-types that was significantly attenuated in KI mice (n=6–7). (b) In mice injected with E0771 cells, there was a significant decrease in the growth and overall weight (inset panel) of tumours in KIs compared with WTs (n=5–7). (c) Less of the endothelial cell marker protein CD31 was observed in PKARI KI tumour tissue compared with WTs detected using immunofluorescence (n=4–5 tumours). (d) The oxidation of PKARI in cultured wild-type mouse aorta treated with VEGF was absent in aorta from littermate knockout Nox4 mice (n=8–11). (e) The enhanced phosphorylation of ERK in wild-type mouse aorta treated with VEGF was absent in aorta from littermate knock-out Nox4 mice (n=6–8). Data are shown as the mean±s.e.m. *P<0.05, **P<0.01 determined by t-test.
