Figure 3: HGF-dependent in vitro angiogenesis is mediated by Arf6.
From: Arf6 regulates tumour angiogenesis and growth through HGF-induced endothelial β1 integrin recycling
(a,b) Angiogenic factor-induced in vitro capillary tube formation by control and Arf6−/− iECs. Scale bar, 50 μm. (c,d) Angiogenic factor-induced microvessel sprouting from aortic ring explants prepared from control and EC-Arf6 cKO mice. Scale bar, 200 μm. (e,f) Time-dependent activation of Arf6 upon HGF (e) and VEGF-A stimulation (stimu) (f). Means±s.e.m. from at least three independent experiments were shown for all panels. *P<0.05; NS, not significant, Student’s t-test for b and one-way analysis of variance with Tukey’s multiple comparison test for d.
