Figure 3: Distinct genome-wide STAT3 binding profile and enhancer landscapes in cDCs and CD4⁺ T cells.

(a–g), cDCs were rested 1 h, treated with IL-21 for 1 h; pre-activated CD4⁺ T cells were washed, rested 16 h, treated with IL-21 for 1 h, and ChIP-Seq performed for STAT3, H3K4me1, and H3K27ac. (a) Venn diagram showing overlapping and distinctive STAT3 binding sites in cDCs and CD4⁺ T cells. (b–g) For IL-21-induced-STAT3 binding sites that differentially exist in cDCs or CD4⁺ T cells, there were cell type-specific binding profiles of STAT3 (b versus c) and H3K4me1 (d versus e) and H3K27ac (f versus g) enhancer marks. Shown are normalized read densities near peak summits for cDC- or CD4⁺ T-cell specific STAT3 binding sites. ‘Dips’ at the plot centres (d–g) represent open chromatin corresponding to nucleosome depletion. Data are representative of two experiments.