Figure 5: Mutational effects on the verapamil-binding site in DinF-BH.
From: Structural basis for the blockade of MATE multidrug efflux pumps
(a) Fluorescence-based measurement of the accumulation of ethidium in bacteria expressing the DinF-BH variants or pET-15b, in the absence (magenta) or presence of 100 μM verapamil (yellow), or 25 μM CCCP (white). (b) The inhibition of bacterial growth as measured by attenuance at 600 nm. Bacteria expressing the indicated DinF-BH variants or pET-15b were grown in the presence of 300 μM verapamil. Error bars indicate s.d. among three biological replicates.
