Figure 6: Structure of verapamil-bound NorM-NG.
From: Structural basis for the blockade of MATE multidrug efflux pumps
(a) Structure of verapamil-bound NorM-NG as viewed from the membrane plane, with the bound verapamil drawn as magenta spheres. The N (residues 5–230) and C (residues 231–459) domains are in cyan and yellow, respectively. (b) Fitting of the asymmetric verapamil to the experimental electron density map (cyan wire), which was calculated to 3.0 Å resolution using density-modified MIRAS phases and contoured at 2.0 σ. The map was overlaid onto the final model of verapamil (magenta sticks). Top view is related to the bottom one by a rotation of 60°. (c) Structural overlay of verapamil- and R6G-bound NorM-NG, as viewed from the periplasmic side. The verapamil-bound NorM-NG is coloured as in (a) while the R6G-bound transporter is in grey. Verapamil (magenta) and R6G (grey) are in stick representation. (d) Close-up view of verapamil-binding site, with verapamil (magenta) and relevant amino acids drawn as sticks.
