Figure 6: Mapping epitopes on ZnuD.
From: The molecular mechanism of Zinc acquisition by the neisserial outer-membrane transporter ZnuD
Since immunization with ZnuD elicits bactericidal antibodies against N. meningitidis, we have mapped antigenic regions on the surface of the ZnuD structures. The zinc/cadmium-bound (a) and the zinc-bound (b) ZnuD structures are used to illustrate the most immunogenic peptides previously identified by Hubert et al.19 from the serum of convalescent patient tested in a ZnuD peptide array experiment. The immunogenic extracellular loops L3, L6 and L11 are coloured in red, gold and blue, respectively. The side (c) and top (d) view of the cadmium/zinc-bound conformation of ZnuD are shown in surface representations to map the localization of the identified immunogenic epitopes.
