Figure 6: EFNB2 and VEGFA are miR-503 target genes. | Nature Communications

Figure 6: EFNB2 and VEGFA are miR-503 target genes.

From: p75NTR-dependent activation of NF-κB regulates microRNA-503 transcription and pericyte–endothelial crosstalk in diabetes after limb ischaemia

Figure 6

(a,b) Luciferase activity at 48 h post-co-transfection of HEK293T cells with either miR-503 or miR-scrambled oligonucleotides (scr oligos) and the following plasmids: 3′-UTR-EFNB2, 3′-UTR-VEGFA, 3′-UTR-EFNB2mut and 3′-UTR-VEGFAmut (with mutation of the putative miRNA target site). **P<0.01 versus scr oligos; ##P<0.01 versus non-mutated (n=5). (c) Representative western blot images and protein quantification of VEGFA and EFNB2). Tubulin was used as a loading control. (d) VEGFA quantification in the medium by ELISA. (e) Proliferation (assessed by 5-bromodeoxyuridine incorporation) or (f) migration of pericytes transfected with miR-503 or siRNA oligos for EFNB2 or VEGFA (control: scr oligos). For c–f, *P<0.05; **P<0.01 versus scr oligos (n=3). Unpaired two-tailed Student’s t-test or Mann–Whitney nonparametric test was applied. All values are mean±s.e.m. of three independent experiments.

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