Figure 8: In vivo transfer of miR-503 during diabetes and ischaemia. | Nature Communications

Figure 8: In vivo transfer of miR-503 during diabetes and ischaemia.

From: p75NTR-dependent activation of NF-κB regulates microRNA-503 transcription and pericyte–endothelial crosstalk in diabetes after limb ischaemia

Figure 8

Unilateral limb ischaemia was induced in diabetic mice and endothelial cells, and pericytes were sorted from the limb muscles using CD31 and NG2 antibodies. Relative expression of pri-miR-503 and mature miR-503 was measured in (a) endothelial cells and in (b) pericytes. (c) Relative expression of EFNB2 and VEGFA in pericytes sorted from the limb muscles. For a–c, *P<0.05, **P<0.01 versus Non Diab (n=10 per group). (d) Quantitative analysis of vascular permeability using Evans blue dye and expressed as ng of dye per mg of muscle tissue (n=8 per group). *P<0.05 versus Non Diab+Ad.Null; #P<0.05 versus Diab+Ad.Null. (e) Representative Isolectin-B4 staining (green) and immunostaining with anti-NG2 antibody (red) in adductor muscle of diabetic ischaemic and diabetic ischaemic mice injected with Ad.decoy503. Scale bar, 100 μm. (f) Quantification of pericyte coverage determined as ratio of NG2 to Isolectin-B4 (Iso-B4) staining. *P<0.05 versus Non Diab+Ad.Null; #P<0.05 versus Diab+Ad.Null (n=6 per group). Unpaired two-tailed Student’s t-test or Mann–Whitney nonparametric test was applied. All values are mean±s.e.m. of three independent experiments.

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