Figure 1: yuc6-1D plants exhibit drought stress tolerance that correlates with reduced ROS accumulation.

(a) Survival assay. The 3-week-old well-watered wild-type (WT, Col-gl), yuc6-1D, 35S:iaaL and yuc6-1DX35S:iaaL plants (top panel) were grown for 9 days under the same conditions but without irrigation, and then photographed (second panel). The drought-stressed plants were then irrigated. At 2 days after resuming irrigation, the plants were photographed (third panel) and the survival rate (per cent) in each sample was quantified as shown at bottom of the picture. (b) Assay of water loss rate. Aerial parts of 3-week-old well-watered soil-grown plants were detached and weighed immediately (0 min). Thereafter, they were allowed to dry naturally under ambient conditions and weighed at the indicated time points. Water content was calculated as the percentage of the fresh weight at time zero. Data represent means±s.e. (n=4). (c) Visualization of H₂O₂ production in response to drought stress. Three-week-old well-watered plants were grown for another 6 day (d) with (−Dry) or without (+Dry) irrigation. Shown are DAB-stained third or fourth rosette leaves of these plants. H₂O₂ production is visualized as a dark brown colour. (d) H₂O₂ accumulations. Aerial parts of 3-week-old plants were detached and air-dried for 4 h (+Dry) or immediately harvested without being air-dried (−Dry). Data represent means±s.e. (n=3). (e) IAA quantifications. Aerial parts of 3.5-week-old plants were used for quantification of IAA as described in Methods. Data represent means±s.e. (n=3).