Figure 4: Cys-85 is not required for the high-auxin phenotype of transgenic plants overexpressing YUC6. | Nature Communications

Figure 4: Cys-85 is not required for the high-auxin phenotype of transgenic plants overexpressing YUC6.

From: A novel thiol-reductase activity of Arabidopsis YUC6 confers drought tolerance independently of auxin biosynthesis

Figure 4

Transgenic lines overexpressing YUC6-YFP (YUC6-OX), YUC6C85S-YFP (YUC6-OXC85S), YUC6mFAD-YFP (YUC6-OXmFAD), YUC6mNADP-YFP (YUC6-OXmNADP), CYP79B2-HA (CYP79B2-OX) and NTRA-HA (NTRA-OX) from the 35S promoter were obtained by transformation of a DR5:GUS transgenic line in the Col-0 background (WT). (a) Western blots show expression of green fluorescent protein (GFP)- and haemagglutinin (HA)-tagged proteins in the transgenic lines. The tagged proteins were detected with anti-GFP and anti-HA antibodies. α-HSP90 and α-tubulin signals are shown as loading controls. (b) One representative 30-day-old transgenic plant of each indicated line is shown (top). GUS staining of 10-day-old seedlings shows elevation of the auxin-responsive DR5:GUS activity only in YUC6-OX, YUC6-OXC85S and CYP79B2-OX plants. (c) IAA quantifications. Aerial parts of 3.5-week-old plants were used for quantification of IAA as shown in Fig. 1e. Data represent means±s.e. (n=3). (d) Expression level of IAA1 and TUBULIN2 (TUB; control) was measured using qRT–PCR in total extracted from 2-week-old plant tissues. Bars represent means±s.e., n=3.

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