Figure 1: Chromatin diffusion is anomalous.
From: Loss of lamin A function increases chromatin dynamics in the nuclear interior
(a) Fluorescence image of a typical nucleus expressing GFP–TRF2 proteins marking telomeres (green) with Hoechst staining (blue). Scale bar, 1 μm. (b) Gray circles show the MSD of an ensemble of simulated diffusing particles for normal diffusion (centre) with α=1, D=2.8 × 10−4 μm2 s−1 compared with anomalous diffusion with α=0.5, D=2.8 × 10−4 μm2 s−0.5 (top right). Grey levels indicate time, with black at τ=0 and lightest gray at τ=1,800 s. Overlaid are two representative trajectories, the colour represents time. The restricted nature of anomalous diffusion is clearly seen. (c) MSD divided by time in U2OS cells as measured for telomeres (black squares, N=958), centromeres (red circles, N=957) and a genomic locus consisting of an integrated lacO array (blue triangles, N=20). (d) MSD divided by time for telomere diffusion in NIH3T3 (black squares, N=325) and HeLa cells (red circles, N=166), all showing anomalous subdiffusion. Symbols designate the average locus MSD while shaded areas mark the s.d. of all single loci MSDs. Individual cell types are presented in Supplementary Figs 1 and 3.
