Figure 2: Lamin A depletion changes chromatin diffusion.
From: Loss of lamin A function increases chromatin dynamics in the nuclear interior
(a) MSDs divided by time for telomeres in Lmna+/+ cells (black squares, N=474), Lmna−/− cells (red circles, N=503) and Lmna−/− cells after transfection with eGFP–pre-lamin A (blue triangles, N=220). Symbols designate the average locus MSD while shaded areas mark the s.d. of all single loci MSDs. (b) Histograms of the anomalous exponent, α, for individual telomeres in Lmna+/+ (black bars) and Lmna−/− cells (grey bars). (c) Histograms of α values of individual telomeres in U2OS cells after siRNA-mediated knockdown of lamin A (black) and control (grey). A clear increase in the anomalous exponent is found (Student’s t-test P<10−10). (d) Depletion of LAP2α in MF cells does not change the anomalous diffusion. Lap2α+/+ telomeres in black squares (N=551) and Lap2α−/− telomeres in red circles (N=629).
