Figure 3: Telomeres scan large areas in lamin A-depleted cells.
From: Loss of lamin A function increases chromatin dynamics in the nuclear interior
The area scanned by 350 randomly selected telomeres during 15 min as characterized by the convex hull in (a) 15 Lmna+/+ cells and (b) 15 Lmna−/− cells. Scanned areas are coloured according to the same logarithmic scale, from 0.01 μm3 (blue) to 10 μm3 (red). (c) Average scanned volumes by telomeres over 15 min in Lmna+/+ (410 loci), Lmna−/− (503 loci) cells and in these same cells transfected with GFP–pre-lamin A (220 loci). Without lamin A, the volume of telomere motion greatly increases. (d) Separation between internal (red) and peripheral (blue) telomeres in a U2OS cell. The convex hull of each telomere is plotted as a solid colour volume and the convex hull of all trajectories is presented as a semitransparent volume. Internal telomeres are defined as those that never touch the total convex hull. (e) Average volume of movement calculated for internal telomeres in Lmna+/+ (232 loci in 20 cells) and Lmna−/− (135 loci in 20 cells).
