Figure 3: Actin and Annexin A2 regulate ATG9A sorting from endosomes.
From: Transcriptional regulation of Annexin A2 promotes starvation-induced autophagy
(a) Colocalization between ATG9A, EEA1 and F-actin in HeLa cells treated with drugs affecting actin polymerization for 2 h; latrunculin A (0.5 μM), jasplakinolide (200 nM) and CK-666 (50 μM). Confocal pictures showing colocalization between ATG9A, EEA1 and F-actin (using Phalloidin staining) are presented with magnified areas. Colocalization between ATG9A and RAB5 (using mRFP-RAB5) in live cells is also shown (Supplementary Movies 2–4). Quantification of ATG9A with F-actin and ATG9A with EEA1 colocalization is shown on the right as Pearson’s coefficient. Data shown as mean ± s.e.m. (n≥20 cells; *P<0.05; two tail t-test). Scale bars, 5 μm. (b) Colocalization between ATG9A, EEA1 and internalized transferrin in Annexin A2 and ARP2 knockdown cells. Confocal pictures showing colocalization between ATG9A, EEA1 and internalized transferrin (30 min) are presented with magnified areas. Colocalization between ATG9A and RAB5 (using mRFP-RAB5) in live cells is also shown (Supplementary Movies 5–7). Quantification of ATG9A and EEA1 colocalisation is shown on the right as Pearson's coefficient. Data are mean ± s.e.m. (n≥20 cells; *P<0.05; two tail t-test). Scale bars, 5 μm.
