Figure 1: Cytoplasmic expression of mouse anti-MBP cyclonals in SHuffle.
From: Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria
(a) Schematic of pET21b-based vector for expression of cyclonals in E. coli. VH, variable heavy; VL, variable light; CH, constant heavy; CL, constant light; RBS, ribosome-binding site. (b) ELISA signals (Abs492) for mouse anti-MBP cyclonals in lysates generated from the following: WT E. coli B strain with a reducing cytoplasm (reducing); isogenic E. coli strains MB1731 and SHuffle engineered with oxidizing cytoplasms (oxi); and, in the case of SHuffle, cytoplasmic DsbC (oxi+cDsbC). The activity measured in MB1731 cells carrying empty pET21b served as a negative control (oxi control). Data are expressed as the mean±s.e.m. of biological triplicates.
