Figure 6: PAR-initiated liquid demixing can filter protein interactions at damaged chromatin.
From: Liquid demixing of intrinsically disordered proteins is seeded by poly(ADP-ribose)
(a) Phase-contrast and fluorescent images depicting that sub-nuclear accumulation of the genome caretaker GFP–53BP1 correlates with decreased light diffraction and reduced levels of Tm-EWS (white arrows), while accumulation of Tm-EWS correlates with increased light diffraction and reduced levels of GFP–53BP1 (black arrows). (b) Movie snapshots from laser microirradiation experiments of GFP-53BP1/Tm-EWS co-expressing cells depicting reduced GFP–53BP1 accumulation at sites of EWS accumulation upon prolonged liquid demixing in PARG-depleted cells. Red arrows point at Tm-EWS accumulation, green arrows point at GFP–53BP1 accumulation. Scale bars, 10 μm.
