Figure 3: Coactivator recruitment by PXR upon co-treatment. | Nature Communications

Figure 3: Coactivator recruitment by PXR upon co-treatment.

From: Synergistic activation of human pregnane X receptor by binary cocktails of pharmaceutical and environmental compounds

Figure 3

(a) Fluorescence anisotropy data showing the relative affinity of the fluorescein-labelled SRC-1 NID for PXR/RXR LBDs heterodimer (20 μM) in the presence of saturating concentrations (60 μM) of TNC, EE2, alone or in mixture, or the PXR agonist SR12813. Assays were performed in three independent experiments and data are expressed as mean (±s.e.m.). (b) Mammalian two-hybrid experiment. Gal4-hPXR and VP16-TIF2 interaction monitored in U2OS cells in presence of DMSO, SR12813, EE2, TNC or EE2+TNC. Assays were performed in duplicate in three independent experiments and data are expressed as mean (±s.e.m.).

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