Figure 3: Transcriptional analysis of CD34 ⁺ DNAM ^bright cells reveals different transcriptional signatures compared with cord blood CD34 ⁺ DNAM ^neg cells.

(a). Transcription factor analysis of CD34⁺DNAM-1^bright cells by RT–PCR. PCR products were separated by electrophoresis on a 1.5% agarose gel and visualized by ethidium bromide staining. CD34⁺DNAM-1^bright cells express Id2, E4BP4, T-bet and FOXP3, while UCMC CD34⁺(DNAM-1⁻) do not express T-bet and FOXP3. (b) Supervised cluster analysis. Supervised cluster analysis was based on the 231 genes differentially expressed (Student’s t-test P value<0.01) between peripheral CD34⁺DNAM-1^bright cells derived from six repeated peripheral blood patient samples (PT) and from two UCMC samples from healthy donors (HD).