Figure 8: Lin − CD34 + DNAM-1 bright cells reside in and can be mobilized from healthy donor BM. | Nature Communications

Figure 8: Lin − CD34 + DNAM-1 bright cells reside in and can be mobilized from healthy donor BM.

From: ‘Emergency exit’ of bone-marrow-resident CD34+DNAM-1brightCXCR4+-committed lymphoid precursors during chronic infection and inflammation

Figure 8

(a) Analysis of healthy bone marrow cells. Flow cytometric analysis of CD3−CD14−CD19−-gated BM cells shows that DNAM-1+CD56− BM cells are CD34+ (upper row). Flow cytometric analysis of Lin−CD34+ cells in BM shows their expression of DNAM-1 on a subset of CD34+ (lower panels). Representative of 10 experiments. (b) DNAM-1brightCD34+ cells are present in the PBMC after HSC mobilization protocols. Flow cytometric analysis of PBMC from HSC apheresis donors (APH) following mobilization protocols (for example, G-CSF without CXCR4 antagonist drugs). DNAM-1 expression is detected on Lin−-gated CD34+ PBMC (upper panel). CD34+DNAMbright-gated APH cells express CXCR4, CD117 and HLA-DR as in CD34+DNAMbright PBMC from HIV patients. Representative of five experiments.

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