Figure 3: Seed coats of atabcg31 and atabcg25 repressed the embryonic growth of aba2-1 less effectively than did those of the wild type (WT).

(a,b) Diagram showing the quantification of the angle between the radicle and the cotyledon and the radicle length, respectively. (a) The angle between the radicle and cotyledon was measured using Axio. (b) Radicle length was measured using Axio. (c–e) Embryos dissected from aba2-1 mutant seeds were placed on a layer of seed coat beddings dissected from the WT (Col-0), atabcg31, atabcg25, atabcg31 atabcg25 (g31/g25-1, g31/g25-2), and atabcg40. The legend in the graph indicates the genotypes of the seeds from which the seed coat beddings were derived. The angle between the cotyledon and radicle (c) and the radicle length (d) of the aba2-1 embryos were measured as indicators of embryonic growth. (c,d) Data are means±s.e.m. of n=108–112 obtained from three independent experiments. Each experiment was performed in duplicate. Each replicate (one SCB) consisted of 20 embryos (N=3, n=2 × 20 embryos). (e) Photographs taken 4 h (top) and 120 h (bottom) after imbibition. Scale bar, 1.25 mm. HAT, hours after transfer. Results shown are representative of three independent experiments (see Supplementary Fig. 7). Each experiment was performed in duplicate. Similar results were obtained in all experiments.