Figure 1: Alternative splicing of HAB1.
From: ABA signalling is fine-tuned by antagonistic HAB1 variants
(a) RT–PCR analysis of the HAB1 transcript levels at 1, 2 and 3 days after germination. ‘M’ indicates DNA molecular weight ladder. (b) Schematic diagram outlining the organization of the HAB1.1 and HAB1.2 variants. The primers used in the a are indicated as P1 and P2. (c) qRT–PCR analysis of the HAB1 variants in different tissues. (d) qRT–PCR analysis of the HAB1 variants at 1, 2 and 3 days after germination with or without 100 μM ABA treatment for 3 h in wild type. GAPDH was used as an internal control. The values were normalized to that of GAPDH. The s.d. of three technical replicates is shown using error bars. Three biological replicates were performed and each gave the similar result.
