Figure 2: The HAB1 variants have opposing functions in the plant ABA response.
From: ABA signalling is fine-tuned by antagonistic HAB1 variants
(a) Schematic diagram showing the constructs used to overexpress HAB1.1 and HAB1.2. The mutated nucleotides are marked in red. (b) RT–PCR analysis to detect the overexpression of HAB1.1 or HAB1.2 in the transgenic lines. RNA was isolated from 7-day-old seedlings. Lane 1: wild type; lane 2: hab1-1; lane 3: hab1-1HAB1.1; lane 4: hab1-1HAB1.2-6; lane 5: hab1-1HAB1.2-8. (c) Western blot analysis of HAB1.2 expression in the transgenic line hab1-1HAB1.2. Total protein was extracted from 7-day-old seedlings, and HAB1.2 was detected using anti-myc antibodies; the star indicates unspecific binding. Lane 1: wild type; lane 2: hab1-1HAB1.2-6; lane 3: hab1-1HAB1.2-8. (d) Seed germination and the development of wild-type, hab1-1, hab1-1HAB1.1, hab1-1HAB1.2-6 and hab1-1HAB1.2-8 plants on MS medium with or without 0.5 μM ABA. The pictures were taken 8 days after germination. (e) Comparison of the germination rates for 5-day-old seedlings (left) and the greening rates for 8-day-old seedlings (right) treated with or without ABA. The s.d. of three experiments is shown using error bars.
