Figure 4: A loss of function of RBM25 disturbs HAB1 AS and alters ABA sensitivity.

(a) Schematic diagram of the T-DNA insertion in the rbm25 mutant. (b) RT–PCR analysis of RBM25 transcript levels in the rbm25 mutant. (c) RT–PCR analysis of the HAB1 variants in rbm25 mutant at 1 and 2 days after germination with or without 100 μM ABA treatment for 3 h. UBQ5 was used as a loading control. (d) qRT–PCR analysis of the HAB1 variants in rbm25 mutant at 1, 2 and 3 days after germination with or without 100 μM ABA treatment for 3 h. GAPDH was used as an internal control. The values were normalized to that of GAPDH. The s.d. of three technical replicates is shown using error bars. More than three biological replicates were performed and each gave the similar result. (e) Phenotypic analysis of wild-type (WT) plants and the rbm25 mutant on MS medium with or without ABA. The pictures were taken 8 days after germination. (f) Comparison of the germination (top) and greening (bottom) rates between WT plants and the rbm25 mutant. The germination and greening rates were scored 5 and 8 days after germination, respectively. The s.d. of three independent experiments is shown using error bars. (g) Root growth in WT and rbm25 plants grown in medium with or without ABA. Five-day-old seedlings from MS medium were transferred to plates with or without ABA and then grown for another 7 days. (h) Quantitative analysis of root growth in WT and rbm25 plants.