Figure 6: RBM25 overexpression rescues the ABA sensitivity of rbm25.
From: ABA signalling is fine-tuned by antagonistic HAB1 variants
(a) qRT–PCR assay of RBM25 expression in WT plants, rbm25 and two transgenic lines harbouring 35S::RBM25-myc construct. RNA was extracted from 7-day-old seedlings germinated on MS medium. The s.d. of three technical replicates is shown using error bars. mRBM25 represents the lines overexpressing RBM25 in the rbm25 mutant. (b) Comparison of the HAB1.2/HAB1.1 ratio in WT plants, rbm25 and two transgenic lines. RNA was extracted from 7-day-old seedlings treated with or without 100 μM ABA for 3 h. (c) Phenotypic analysis of WT and rbm25 plants and two transgenic lines grown on MS medium with or without ABA. The pictures were taken 8 days after germination. (d) Comparison of the germination and greening rates among WT plants, rbm25 and two transgenic lines. The germination and greening rates were scored 5 and 8 days after germination, respectively. The s.d. of three independent experiments is shown using error bars. (e) Root growth in WT plants, rbm25 and two different lines grown in medium with or without ABA. Five-day-old seedlings from MS medium were transferred to plates with or without ABA and grown for another 7 days. (f) Quantitative analysis of root growth in WT plants, rbm25 and two transgenic lines in response to ABA.
