Figure 7: RBM25 binding to the last intron of HAB1.
From: ABA signalling is fine-tuned by antagonistic HAB1 variants
(a) RBM25 is induced by ABA slightly. Ten-day-old seedlings germinated on MS medium were treated without or with 100 μM ABA for 3 h, and total RNA was used for qRT–PCR analysis. (b) The construct harbouring gRBM25-GFP was transformed into the rbm25 plants, and the fluorescence of RBM25-GFP was observed in the leaf cells of 2-week-old seedlings. The nucleus localization of RBM25 was confirmed by DAPI staining. Scale bar, 20 μm. (c,d) Interaction analysis between RBM25 and U1-70K using yeast two-hybrid assay (c) and BiFC analysis (d).(e) RIP assay showing that RBM25 binds to the last intron of HAB1. Input protein was 10% of total input proteins. The amounts of HAB1.1, HAB1.2-3 and HAB1.2-5 fragments were determined by qRT–PCR and normalized to the input. HAB1.1, HAB1.2-3 and HAB1.2-5 were amplified using the primer pairs of F1/R1, F2 /R2 and F3/ R3, respectively. The s.d. of three biological replicates is shown using error bars (t-test, *P<0.05).
