Figure 2: Molecular cloning of ROA1. | Nature Communications

Figure 2: Molecular cloning of ROA1.

From: An Arabidopsis PWI and RRM motif-containing protein is critical for pre-mRNA splicing and ABA responses

Figure 2

(a) Map-based cloning of ROA1. Structure of the ROA1 gene and positions of the roa1-1 and roa1-2 mutations are indicated. Filled boxes and empty boxes indicate exons and untranslated regions, respectively; the solid lines between boxes indicate introns. Positions are relative to translation start sites. (b) Structure of ROA1. Motifs are predicted by the SMART database. (c) Phylogenetic tree of RBM25 and its close orthologues from other organisms. The protein identities are the same as defined in Supplementary Fig. 2. The Bootstrap consensus tree was generated with MEGA6 (ref. 69). Protein sequences of RBM25 and its orthologues were aligned by MUSCLE and the Bootstrap consensus phylogenetic tree was reconstructed by the statistical method maximum likelihood using the Jones–Taylor–Thornton (JTT) model with a Bootstrap of 1000. Cut-off value for consensus tree is 50%. (d) Gene complementation analysis. (e) Chlorophyll content in plants shown in d. (f) Relative transcript levels of ROA1 in WT and roa1-1 plants as determined by qRT–PCR analysis. (g) Nuclear localization of the RBM25–GFP fusion protein. Scale bars, 2.5 μm. Values represent mean±s.d. (n=8 in e; 4 in f).

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