Figure 3: Intestinal E. coli in mice.

Relative abundance of E. coli was determined by real-time PCR using specific uidA primers and quantified using the 2^−▵Ct method. (a) Relative abundance of E. coli in groups of 6-week-old WT and ST mice treated with the antibiotics vancomycin (Van) and streptomycin (Stp) and challenged with 3% DSS. Mice were treated for 8 days with or without antibiotics and DSS for the first 5 days in drinking water. Control mice received sterile drinking water. (b) Relative abundance of E. coli in 6-week-old WT, ST and respectively cross-fostered (XF) mice, which were fed by foster mothers of the other genotype during lactation. The data are represented as median values. Each point indicates a single mouse from two independent experiments. N=6–8, *P<0.05 (ANOVA, Bonferroni’s multiple comparison test).