Figure 2: Growth kinetics and HA titres of HY#1–7 high-yield candidates in Vero cells.
From: Development of high-yield influenza A virus vaccine viruses
(a) Growth kinetics and HA titres of high-yield candidates in Vero cells. Vero cells were infected in triplicate with the indicated viruses at a multiplicity of infection (MOI) of 0.005 and incubated at 37 °C. Supernatants were collected at the indicated time points, and the virus titres were determined by plaque assays in MDCK cells. In parallel, we determined the HA titres of the collected supernatants by performing HA assays. (b) Effect of the C4U promoter mutation in the viral polymerase genes on viral growth kinetics and HA titres. Shown is the comparison of viruses possessing the parental UW-PR8 backbone (UW-PR8_Indo09), the HY#1 backbone (HY#1_Indo09), or the HY#1 backbone with C4U mutations in the PB2, PB1 and PA genes (HY#1+C4U_Indo09). Experiments were carried out as described in a. The values presented are the average of three independent experiments±s.d.
