Figure 6: Evaluation of PR8-HY vaccine candidate viruses propagated in embryonated chicken eggs.
From: Development of high-yield influenza A virus vaccine viruses
Growth kinetics and HA titres of UW-PR8- and PR8-HY-based viruses encoding the wild-type, or wild-type and chimeric HA and NA segments of the A/chicken/Indonesia/NC/2009 (Indo09, H5N1) (a), A/Vietnam/1203/2004 (VN04, H5N1) (b), A/Hubei/1/2010 (Hubei10, H5N1) (c), A/Egypt/N03072/2010 (Egypt10, H5N1) (d), A/Indonesia/5/2005 (Indo05, H5N1) (e), or A/Anhui/1/2013 (Anhui13, H7N9) (f) viruses. Panels (g) and (h) show a comparison of current seasonal H1N1 and H3N2 vaccine viruses (X-181 and X-223A, respectively) with PR8-HY backbone viruses possessing wild-type or chimeric HA and NA segments derived from X-181 or X-223A viruses. Ten-day-old embryonated chicken eggs (four per virus) were inoculated with 2 × 103 PFU of the respective viruses and incubated at 35 °C for the indicated periods of time. The values presented are the average of three independent experiments±s.d.
