Figure 5: DODS applied to macrophages. | Nature Communications

Figure 5: DODS applied to macrophages.

From: Measuring fast stochastic displacements of bio-membranes with dynamic optical displacement spectroscopy

Figure 5

(a) Fluorescence image and measurement positions (white dashed lines) for a macrophage, scale bar, 5 μm. (b) Displacement traces, δh, (shifted by +0.5 μm for clarity) and dACFs (cell body (body)/lamellipodium (lam), IFNγ+/IFNγ−). (c) fluctuation amplitude, , and relaxation time, τ*. Error bars denote s.d. (d) Histogram of displacements, 〈N(δh)〉, and deviation from a Gaussian fit, 〈ΔN(δh)〉, averaged for all cells (six different IFNγ+ experiments (lam: N=29 and body: N=11) and five different IFNγ− experiments (rim: N=26 and centre: N=26)). Significance levels for differences in skewness (S) and kurtosis (K) are indicated. Significance according to Mann–Whitney U-test P<0.001 (***), P<0.01 (**) and P<0.05 (*).

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