Figure 1: Induction of T-cell recruitment and inflammatory demyelination by a single fibrinogen injection in the CNS. | Nature Communications

Figure 1: Induction of T-cell recruitment and inflammatory demyelination by a single fibrinogen injection in the CNS.

From: Blood coagulation protein fibrinogen promotes autoimmunity and demyelination via chemokine release and antigen presentation

Figure 1

(a) Demyelination (LFB/PAS and toluidine blue), microglial activation and demyelination (MBP/Iba-1), and T-cell infiltration (CD3) in the corpus callosum of mice injected with fibrinogen compared with ACSF control. Scale bar, 100 μm (top panel); 10 μm (second panel); 100 μm (third panel); 80 μm (bottom panel). Representative histological sections from day 7 after injection are shown. Data are presented as mean±s.e.m. (n=5–6 mice per time point). *P<0.05, ***P<0.001, ****P<0.0001 (two-way ANOVA and Bonferroni’s multiple comparisons test). (b) FACS analysis of T cells isolated from the brain (corpus callosum) 7 days after fibrinogen or ACSF injection stained with CD3, CD4 and CD8 (n=3 independent experiments; each experiment generated from pooled brain cells from n=3–4 mice). ANOVA, analysis of variance; d, days; FACS, fluorescence-activated cell sorting; LFB, Luxol fast blue.

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