Figure 7: Genetic inhibition of CD11b blocks fibrinogen-induced microglial activation and inflammatory demyelination. | Nature Communications

Figure 7: Genetic inhibition of CD11b blocks fibrinogen-induced microglial activation and inflammatory demyelination.

From: Blood coagulation protein fibrinogen promotes autoimmunity and demyelination via chemokine release and antigen presentation

Figure 7

(a) Quantification of microglial activation (IBA-1), T-cell infiltration (CD3) and demyelination (LFB/PAS) 7 days after fibrinogen injection in the corpus callosum of Itgam−/− or control WT mice (n=6). Data are presented as mean±s.e.m. *P<0.05, **P<0.01 (non-parametric Mann–Whitney U-test). (b) Fibrinogen-induced gene expression of Cxcl10 and Ccl2 is reduced in the corpus callosum of Itgam−/− mice compared with WT control. Results are mean±s.e.m. of 6–7 mice per group, **P<0.01, ****P<0.0001 (two-way ANOVA and Bonferroni’s multiple comparisons test). (c) Fibrinogen-induced gene expression of T-bet, IFN-γ and IL-12p40 is reduced in the corpus callosum of Itgam−/− mice compared with WT control (n=5–8 mice). Data are presented as mean±s.e.m. **P<0.01, ***P<0.001 (two-way ANOVA and Bonferroni’s multiple comparisons test). ANOVA, analysis of variance; d, days; LFB, Luxol fast blue.

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