Figure 1: Isolation of stable ALT survivors in C. elegans with distinct telomeric sequences.
From: Telomere maintenance through recruitment of internal genomic regions
(a) A schematic diagram showing the experimental procedures to isolate stable ALT survivors in two wild isolates. (b) Quantitative FISH analysis for telomere length of the survivors CB4856 trt-1, pot-2(tm1400), CS1 and CS2. Telomere was detected by Cy3-TTAGGC*3 PNA probe (red) in the embryo. DNA was counterstained with 4′,6-diamidino-2-phenylindole (blue). The upper panel shows representative images for each strain. t-test was used for statistical analysis for quantification (*P value <0.0001, n=388/each). Mean value is represented with red bar. Scale bar, 10μm. (c) TRF analysis of N2, CB4856, pot-1(tm1620), pot-2(tm1400) and all the CS survivors digested with a combination of six-cutter restriction endonucleases (NheI, DraI, ApaI, NdeI, XhoI, NcoI and SacI) and probed with DIG-TTAGGC*4. pot-1(tm1620) and pot-2(tm1400) were used as positive controls as they have long telomeres. (d) TRF analysis of CB4856, CB4856 trt-1 and all the CS survivors probed with DIG-TTAGGC*4 after HinfI digestion.
