Figure 3: TALT1 is replicated in the telomeres of CB4856 survivors.
From: Telomere maintenance through recruitment of internal genomic regions
(a) Diagram illustrating how the contigs (red bar) were reconstructed from reads in which one pair aligned to TALT1. Asterisks indicate predicted breakpoint by BLAST (black) or confirmed breakpoints (red). (b) Confirmation of trans-duplication by breakpoint PCR followed by Southern hybridization with TALT1 probe. Breakpoint PCR primers (red arrows) were designed to amplify the junction of TALT1 and each of proximal telomere regions. F, forward primer; R, reverse primer; open arrow, telomere repeat. (c) FISH using the TALT1 probe and telomere probe in embryonic stage. Scale bar, 10μm; blue, 4′,6-diamidino-2-phenylindole; green, TALT1; red, telomere. (d) TRF analysis using a TALT1 probe and a telomere probe. DNA was digested with indicated enzymes and hybridized with indicated probes. RE, restriction endonuclease; C, CB4856 trt-1; S, CS1 survivor; H, HindIII; B, BamHI; Nc, NcoI; Nd, NdeI; Nh, NheI; S, SacI; Mix, mixure of HindIII, BamHI, NcoI, NdeI, NheI and SacI.
