Figure 4: NS survivors amplify and incorporate TALT2 in their telomeres.
From: Telomere maintenance through recruitment of internal genomic regions
(a) TRF analysis for telomere and TALT2 of N2 survivors. Genomic DNA of N2 trt-1 and NS survivors (NS1–NS5) was digested with HinfI or AluI. The blot was hybridized with (TTAGGC)*4 repeat (upper panel). The same blot was stripped and reprobed with a TALT2-specific probe (bottom panel). Arrowheads indicate cleaved TALT2. (b) Fold change from average read depth plot (black bar) of N2 trt-1 and N2 survivors. To normalize strain-specific variation, fold change from average depth from parental N2 trt-1 was subtracted from total fold change (grey bar) of ALT survivors. The bottom panel is an enlargement of TALT on chromosome (Chr) I. Internal telomere repeats are indicated as black bars. (c) FISH using the TALT and telomere probe in embryo of CB4856 trt-1, N2 trt-1 and all the survivors. Scale bar, 10μm; blue, 4′,6-diamidino-2-phenylindole; green, TALT; red, telomere.
