Figure 5: trans-Duplication of TALT1 reservoir to other chromosomal ends in CB4856 backgrounds.
From: Telomere maintenance through recruitment of internal genomic regions
(a) Fold change from average coverage of SNVs (WBvar00067712 and WBvar00067717) from WGS data of N2 trt-1, CB4856 trt-1, CS1 and CS2. N2-associated haplotype (blue) and CB4856-associated haplotype (red) are plotted separately. Owing to large difference of value, y axis is segmented. (b) ‘CB4856-derived’ SNVs were located at the right end of chromosome V in CB4856, but not in N2. Asterisks indicate CB4856 SNVs. Dashed line indicates genomic position. (c) Breakpoint PCR was performed with primer pairs (red arrows in b) designed to anneal to the proximal telomere region of chromosome V (VR) and TALT1 in forward (For in b) or reverse (Rev in b) orientation. CB, CB4856. (d) Nucleotide change of WBvar00067712 and WBvar00067717 in internal and telomeric TALT1. N2-associated alleles are blue while CB-associated alleles are red. Nucleotide positions are indicated in d.
