Figure 3: CRISPR/Cas9-induced morphological mutants of Abdominal-B, ebony and frizzled in P. xuthus.
From: Outbred genome sequencing and CRISPR/Cas9 gene editing in butterflies
(a–d) Mutations induced by the injection of Cas9 mRNA and Abdominal-B sgRNA with prolegs on segment A7–A10 (a), segments that do not normally have prolegs (c), or with a curled abdomen resulting from abnormal terga on segments A3 and after (b), compared with wild type (d). Green dots in a and c show normal prolegs of A6 and A10, while red stars in a denote the redundant prolegs on A7–A10. (e–j) Mutations induced by the injection of Cas9 mRNA and ebony sgRNA with enhanced melanic pigmentation (e) and an absence of orange colour in the false eyespot (f) in fifth instar larvae, compared with wild type (h,i), and with brown pigmentation (g) across the body in regions that were normally yellow and in wing patches that were normally orange in adult (j). (k–n) A mutant induced by the injection of Cas9 mRNA and frizzled sgRNA with smooth and colourless dorsal cuticle in right side (k), while normal in its left side (m), and its dorsal view (l) compared with wild type (n). Scale bars, 1 mm (a–d,f,i,k–n) and 10 mm (e,g,h,j).
