Table 2 Mutagenesis efficiency of knocking out genes Abdominal-B, ebony and frizzled in P. xuthus.

From: Outbred genome sequencing and CRISPR/Cas9 gene editing in butterflies

Gene

Target sites*

RNA con. (ng μl−1)†

Phynotype rate (%) ‡

Mutation rate (%)

Mutation type

Px_03961_Abd-B

T42

200; 300

7.95 (7/88)

35.38

D: 3–51; I: 4–18

  

600; 600

8.26 (10/121)

25

D: 3–66; I: 4–5; M: 40

 

T42/T95

50/50; 100

6 (6/100)

18.33

D: 5–59; I: 3–19

  

566/416; 1,200

91.79 (123/134)

90.85

D: 3–73; I: 3–64; M: 3–29

Px_01073_e

T2/T303

158/159; 1,200

31.25 (5/16)

66.67

D: 5–33; I: 3–10; M: 3

 

T454/T6

200/150; 1200

88 (22/25)

29.92

D: 6–62; I: 3–15; M: 3–8

Px_15230_fz

T268/T283

500/500; 1,,000

4.12 (4/96)

45.16

D: 3–64; I: 3–15; M: 4–5

  1. D, delete fragment (bp); I, insert fragment (bp); M, mutated fragment (bp); sgRNA, single guide RNA.
  2. *‘/’ denotes injection of mixed targets.
  3. †The number before and after semicolon denotes injected concentration of sgRNA and Cas9 mRNA, respectively.
  4. ‡The number in the bracket denotes mutant/observed injected individuals.
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