Figure 5: XPA protein is required for 6–4PP removal from telomeres. | Nature Communications

Figure 5: XPA protein is required for 6–4PP removal from telomeres.

From: Telomeres are partly shielded from ultraviolet-induced damage and proficient for nucleotide excision repair of photoproducts

Figure 5

(a,b) Cells were exposed to 10 J m−2 UVC and harvested at various repair times (0, 1.5 and 3 h, experiment a; 0 and 12 h, experiment b). Telomeres were isolated from purified genomic DNA (200 μg each time point) to obtain 13–14 ng (lane 3, a; lane 2, b) for loading. Genomic DNA was loaded at amounts indicated. The blots were sequentially probed with a 6–4PP antibody, a radiolabelled telomere probe, and a radiolabelled Alu repeat probe. (c) The 6–4PP signal intensity was quantitated, normalized to 0 h, and plotted against recovery time. Genomic DNA values represent the mean and s.e.m. from four independent experiments, and values and error bars for telomeres were derived from two independent experiments for each time point. The difference between the curves was not statistically significant (P=0.17) by two-way ANOVA.

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