Figure 1: PE-containing vesicles are closely associated with the nuclear envelope.
From: Nuclear envelope-associated endosomes deliver surface proteins to the nucleus
(a) Confocal imaging of PE+ vesicular structures in MG63 cells stained with the ER marker PDI and the DNA intercalating dye 4,6-diamidino-2-phenylindole (scale bar, 25 μm). Image is representative of 10 independent experiments. (b) Deconvolution microscopy image demonstrating close association of PE+ structures with the nuclear envelope as revealed by fluorescent staining of the envelope components lamin A and C (scale bar, 5 μm). (c) Z/X reconstruction image demonstrating close association of PE+ structures with the lamin sheet (scale bar, 5 μm). (d) 3D-SIM super-resolution microscopy image of PE and lamin A/C (scale bar, 1 μm). Image is representative of three independent experiments.
