Figure 2: Vesicles associated with the nuclear envelope are derived from early endosomes.
From: Nuclear envelope-associated endosomes deliver surface proteins to the nucleus
(a) Cells pre-treated with Brefeldin A (BFA) or dimethylsulfoxide (DMSO) both before and during incubation with Alexa 488-PE were stained for giantin (scale bar, 5 μm). (b) Percentage of cells displaying NAE structures. Representative of three independent experiments (error bars indicate s.d.). (c) Average number of NAE structures by nuclei. Representative of three independent experiments (error bars indicate s.d.). (d) Co-labelling of PE with early endosomal marker EEA1. (e) Co-labelling with the late endosomal marker Rab7 (scale bar, 10 μm, × 5 image: scale bar, 2 μm). Data shown in c and d are from three experiments.
