Figure 3: NAE display low motility, frequent fusion events and progressive loss of vesicular content.
From: Nuclear envelope-associated endosomes deliver surface proteins to the nucleus
(a) MG63 cells were incubated for 1 h with Alexa 488-conjugated PE and then imaged every 0.5 s for a total duration of 5 min. (b) Quantification of vesicle velocity. (c) NAE number over time in an individual live cell. (d) NAE frequency in six individual cells. (e) Apparent NAE fusion event. (f) Increase in PE fluorescence intensity consistent with NAE fusion. Images were taken every 5 s for a total duration of 3 h. (g,h) Decreasing fluorescence intensity of PE-laden NAEs over time suggesting progressive loss of vesicular contents (scale bar, 10 μm). Images and data are representative of six independent experiments.
