Figure 3: Immunogenetic analysis showed a germline heavy chain with a unique junctional motif. | Nature Communications

Figure 3: Immunogenetic analysis showed a germline heavy chain with a unique junctional motif.

From: Junctional and allele-specific residues are critical for MERS-CoV neutralization by an exceptionally potent germline-like antibody

Figure 3

(a) m336 VH, VL sequence alignments with germline gene segments are shown. Identical residues are denoted by dots and junction residues at HCDR3 are boxed. Antibody residues are numbered according to the Kabat nomenclature. (b) Close-up view of interactions between the junctional amino acids in the HCDR3 of m336 with MERS-CoV RBD. (c) Amino acid sequences of HCDR3s that were found similar to that of m336 from 454 sequencing analysis of IgM libraries derived from 69 healthy human subjects (denoted by five-letter codes), and from the report by Tang et al.28 (3B11 and 3B12). Red boxes show the junction amino acid residues in HCDR3s. (d) Germline-rooted circular phylogenetic tree of m336 VL-like antibody sequences (denoted by five-letter codes, m336 in red and KV1-17*01 in green) found in IgM libraries derived from 69 healthy human subjects and two newborn babies along with the anti-rabies antibody light chain (SC4098, in purple) from the report by Kramer et al.38.

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