Figure 4: The binding and neutralization activities. | Nature Communications

Figure 4: The binding and neutralization activities.

From: Junctional and allele-specific residues are critical for MERS-CoV neutralization by an exceptionally potent germline-like antibody

Figure 4

(a) Representation of the amino acids in m336 that are divergent from those in the predicted germline precursor. None of the five residues in the light chain has any direct contact with the MERS-CoV RBD. (b) Binding of IgG1s m336, m336-gH, m336-gL-FR, m336-gL and a negative control IgG1 m102.4 to MERS-CoV S1 protein measured by ELISA. (c) Neutralization of viruses pseudotyped with the MERS-CoV S glycoprotein. (d) A summary of the binding and neutralization results of m336 and the germline revertants. (e) Binding of Fab m336 allele-specific mutants (F54L and K73E) to MERS-CoV S1 protein measured by ELISA. (f) Binding of Fab m336 junctional mutants (V95A, N100dA, R100eA and G100fA) to MERS-CoV S1 protein measured by ELISA.

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