Figure 6: The Pfn2–HDAC1 interaction is dependent on their C terminus.
From: Epigenetic regulation of Smad2 and Smad3 by profilin-2 promotes lung cancer growth and metastasis
(a) Co-immunoprecipitation of Pfn1 and Pfn2 with HDAC1 and HDAC2. (b) Schematic illustration of full-length and deleted Pfn2 and HDAC1 proteins. NLS represent the nuclear localization signal. (c) The interaction between myc-tagged HDAC1 and flag-tagged Pfn2 fragment was detected by co-immunoprecipitation. (d) The expressions of Smads and HDACs were detected by western blotting. (e) The cellular localization of HDAC was detected by western blotting of the extracts of cytoplasmic and nuclear portions of cells. PARP was used as a marker of nucleus and tubulin was used as a marker of cytoplasm. (f) The interaction between flag-tagged Pfn2 and myc-tagged HDAC1 fragment was detected by co-immunoprecipitation. For all the western blotting results in this figure, one representative result of three experiments is shown.
