Figure 7: The effect of HDAC1 on Smad expression.
From: Epigenetic regulation of Smad2 and Smad3 by profilin-2 promotes lung cancer growth and metastasis
(a–c) The binding of HDAC1 to Smad promoter was detected by ChIP assay. Student’s t-test is used for comparison of vector versus Pfn2 and ANOVA test for comparison of scramble, shPfn2-1 and shPfn2-2; all the P values are shown in the figure. Error bars represent the means±s.d. of three independent experiments. (d) Smad expression was detected by western blotting. One representative result of three experiments is shown. (e) The relative soft agar colony formation abilities were shown. ANOVA test; P=0.0003. Error bars represent the means±s.d. of four independent experiments. (f) The matrigel invasion ability was determined by the number of invaded cells. ANOVA test; P=0.0028. Error bars represent the means±s.d. of three independent experiments. (g–i) The binding of HDAC2 to Smad promoter was detected by ChIP assay. ANOVA test; all the P values are shown in the figure. Error bars represent the means±s.d. of three independent experiments. (j,k) The dose- and time-dependent effects of TSA on Smad expression. One representative result of three experiments is shown. ANOVA, analysis of variance.
