Figure 7: Regulation of the binding of C/EBPβ to the EDIL3 promoter.

(a) ChIP analysis of C/EBPβ binding to EDIL3 promoter in HUVEC treated with medium only, RvD1 (100 nM) or IFNγ (10 ng ml⁻¹). After 4 h incubation, cross-linked chromatin was immunoprecipitated with anti-C/EBPβ IgG, non-immune IgG (negative control) or with anti-Histone H3 IgG (positive control). Immunoprecipitated chromatin was subjected to PCR using specific primers designed to amplify the −328 to −589 bp region of the EDIL3 promoter that contains C/EBPβ binding sites. PCR products were visualized by gel electrophoresis and staining with SYBR safe gel dye. (b) Similar experiment in which cross-linked chromatin was immunoprecipitated with anti-KLF4 IgG. (c) ChIP analysis of C/EBPβ binding to EDIL3 promoter in HUVEC which were treated or not with IL-17 (10 ng ml⁻¹; 4 h) with or without 30 min pre-treatment with RvD1 (100 nM). In experiments using LY249002 (20 μM; PI3K inhibitor) or LY303511 (20 μM, inactive analogue), these compounds were added 1 h before RvD1. IP, immunoprecipitation.