Figure 9: RvD1 inhibits ligature-induced bone loss.

Periodontal bone loss was induced for 5 days in wild-type (a–c) or Del-1-deficient (d) mice by ligating a maxillary second molar and leaving the contralateral tooth unligated (baseline control). The mice were locally microinjected into the palatal gingiva with IL-17 (0.2 μg), RvD1 (0.2 ng), or their combination, 1 day before placing the ligature and every day thereafter until the day before sacrifice (day 5). Ligature-induced periodontal inflammation in wild-type mice was monitored in dissected gingival processed for determining MPO (a measure of neutrophil infiltration) by ELISA (b) or for qPCR to determine mRNA expression of the indicated molecules, normalized against GAPDH mRNA (c). The data are expressed as fold change in the transcript abundance in the ligated side relative to that of the unligated side, which was assigned an average value of 1. Data are means±s.d. (n=5 mice). *P<0.05 and **P<0.01 between indicated groups (unpaired t-test).