Figure 4: Identification of the active surface of RhTx.
From: A pain-inducing centipede toxin targets the heat activation machinery of nociceptor TRPV1
(a) Calcium imaging of mTRPV1-expressing HEK293 cells challenged by 10 μM RhTx point mutant (D20A), 10 μM RhTx, 10 μM capsaicin and 1 mM ionomycin, respectively. Scale bar, 100 μm. (b) Representative trace of calcium signal. (c) Comparison of mTRPV1 responses induced by RhTx mutants (1 μM each) to that induced by 1 μM wild-type toxin and 10 μM capsaicin. The holding potential of this whole-cell recording was 0 mV and testing potential was +80 mV. (d) EC50 values of WT RhTx and mutants. **P<0.001 (n=3–8). (e) CD spectra of WT RhTx and mutants exhibit no significant difference. EC50, effector concentration for half-maximum response.
