Figure 2: LRF-deficient cells are hypersensitive to ionizing radiation.
From: LRF maintains genome integrity by regulating the non-homologous end joining pathway of DNA repair
(a–c) Clonogenic survival of Arf−/− and Arf−/− LRF-deleted MEFs treated with γ-radiation (a), phleomycin (b) and mitomycin C (c). Data from n=4 independent experiments are presented as mean±s.e.m. Associated P value calculated by Student’s t-test analysis is indicated. (d) DNA damage levels in Arf−/− and Arf−/− LRF-deleted MEF treated with phleomycin assessed by comet assay. The percentage of DNA in comet tails is scored from 200 cells of three different experiments and presented as mean±s.e.m. Associated P value calculated by Student’s t-test analysis is indicated. (e) γ-H2AX levels assessed by flow cytometric analysis of Arf−/− and Arf−/− LRF deleted MEFs, 1 h after 20 or 300 μM phleomycin treatment. Data from n=4 independent experiments are presented as mean±s.e.m. Associated P value calculated by Student’s t-test analysis is indicated. (f) Survival curve of Lrf hematopoietic system conditional knockout mice (Lrf cKO) (n=6) and sibling control mice (n=9) after single dose of whole-body γ-radiation (7.5 Gy).
